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KMID : 1134820170460091143
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 9 p.1143 ~ p.1150
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Comparison of Quantitative Structure-Activity Relationship and Chemical Antioxidant Activity of β-Carotene and Lycopene and Their Protective Effects on Intracellular Oxidative Stress
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Park Sun-Young
Jung Ha-Na
Jhin Chang-Ho
Hwang Keum-Taek
Kwak Ho-Kyung
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Abstract
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The aim of this study was to determine the chemical and intracellular antioxidant activities of β-carotene and lycopene and to compare their quantitative structure-activity relationship (QSAR). In our previous study, the second ionization energy of lycopene was higher than that of β-carotene, as calculated by QSAR. Chemical antioxidant activities of β-carotene, lycopene, and Trolox were examined by measuring ferric reducing antioxidant power (FRAP) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Intracellular antioxidant activities were evaluated by intracellular reactive oxygen species (ROS) and DNA fragmentation. The FRAP of lycopene was higher than that of β-carotene (P<0.05), and the two carotenoids had similar antioxidant activities in DPPH radical scavenging activity assay. Trolox had the greatest chemical antioxidant activities (P<0.05). When RAW264.7 cells were treated with lipopolysaccharide (LPS) (100 ng/mL) for 20 h, intracellular ROS and DNA fragmentation significantly increased (P<0.05). RAW 264.7 cells pretreated with β-carotene (4 μM) and lycopene (0.4∼2 μM) for 4 h formed significantly less intracellular ROS than LPS-treated control cells (P<0.05), whereas cells with Trolox did not reduce production of intracellular ROS. In addition, cells pretreated with 2 μM lycopene produced less intracellular ROS than those treated with β-carotene (P<0.05). DNA fragmentation of cells with β-carotene and lycopene was similar to that of LPS-treated control cells as measured by Hoechst staining. The antioxidant ability of lycopene was greater than that of β-carotene in the QSAR, FRAP, and intracellular ROS assays (P<0.05). β-Carotene and lycopene had lower antioxidant activities as measured by FRAP (P<0.05) but higher intracellular protective effects against LPS-induced oxidative stress in comparison with Trolox.
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KEYWORD
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β-carotene, lycopene, antioxidant activity, lipopolysaccharide, reactive oxygen species
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